Genetic characterization of Salmonella enterica serovar Newport isolated from cattle using a DNA microarray to identify genes conveying multi-drug resistance, increased virulence and conjugative ability
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Characterization of Salmonella enterica serovar Typhimurium conjugative plasmids transferring resistance to antibiotics and their interaction with the virulence plasmid.
OBJECTIVES In this study, we analysed field isolates of Salmonella enterica serovar Typhimurium for the presence of conjugative plasmids transferring resistances to antibiotics. METHODS Altogether 23 strains were analysed for the presence of conjugative R-plasmids. In the case of successful conjugation, the R-plasmids were characterized by PCR for antibiotic resistance genes, integrons and re...
متن کاملComplete Closed Genome Sequences of Salmonella enterica subsp. enterica Serotypes Anatum, Montevideo, Typhimurium, and Newport, Isolated from Beef, Cattle, and Humans
Salmonella enterica spp. are a diverse group of bacteria with a wide range of virulence potential. To facilitate genome comparisons across this virulence spectrum, we present eight complete closed genome sequences of four S. enterica serotypes (Anatum, Montevideo, Typhimurium, and Newport), isolated from various cattle samples and from humans.
متن کاملIdentification and functional analysis of Salmonella enterica serovar Typhimurium PmrA-regulated genes.
The PmrA-PmrB two-component regulatory system of Salmonella enterica serovar Typhimurium is activated in vivo and plays an important role in resistance to cationic antimicrobial peptides. Resistance is partly mediated by modifications to the lipopolysaccharide. To identify new PmrA-regulated genes, microarray analysis was undertaken comparing cDNA derived from PmrA-constitutive and PmrA-null st...
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BACKGROUND Many Gram-positive and Gram-negative bacteria produce large quantities of indole as an intercellular signal in microbial communities. Indole demonstrated to affect gene expression in Escherichia coli as an intra-species signaling molecule. In contrast to E. coli, Salmonella does not produce indole because it does not harbor tnaA, which encodes the enzyme responsible for tryptophan me...
متن کاملOccurrence of multidrug-resistant Salmonella enterica serovar Enteritidis isolates from poultry in Iran
Salmonella enterica is recognized as one of the major food-borne pathogens with more than 2,500 serotypesworldwide. The present study addresses antimicrobial resistance of Salmonella enterica serovar Enteritidisisolates in Iran. A collection of 151 Salmonella spp. isolates collected from poultry were serotyped toidentify Salmonella Enteritidis. Sixty-one Salmonella Enteritidis were subsequently...
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